PicoTechnology for Biorationals and BioPesticides PestControls.
805 Cottage Hill Way, Brandon, Fl 33511
USA 800-995-9203, Intl 336-306-0193
Email or Call: donwilshe@biobased.us

Science suggests that "PicoAg 4N1 25B" can be mechanical in primary sequential steps!

Bacteria's Fungi's Virus's Insects's Nursery Tours's
Picotechnology-Industries Pico-Medicine Picocides Pico-Technology Picosoap

World Speaking Invitations, To present our work on Picotechnology 1000 times smaller than Nano! PicoTechnology vs Nanotechnology
1) Dubai Speaker: 3rd World Congress & Expo on Nanotechnology and Materials Science during March 25-26, 2019 Dubai, UAE PicoTechnology vs Nanotechnology
2) Spain Speaker: World Chemistry Forum Nano Science Technology, May 22-24, 2019 in Barcelona, Spain. PicoTechnology vs Nanotechnology

In 2016 we had 2 agriculture journals papers published,and working on 2 more now!

Ramesh Ravella Sweet Sorghum 1312015AJEA23608 Biofuel Feedstock Cut fertilizer by 50% and increased crop production!
Ramesh Ravella Canola Bio-Material Analyses of Two Canola Cultivars Cut fertilizer by 50% increased crop production!

Lets Revisit "Biorationals" and "Biopesticides", No Side Effects, Biologicals, Organic Chemistry, Graphene or Nanotechnology, It's Pico Time!
This was our first and last attempt in 2008 at these Biopesticides pests and we should of increased the rate to 5oz/10gallon acre application program, but did very well!

We dont see these buys as Pico competition, The biologicals buying spree by agchem companies large and small swept the industry almost as fast as the spread of weed resistance. Bayer’s trendsetting purchase of AgraQuest for nearly $500 million to BASF’s $1.02 billion acquisition of Becker Underwood to Monsanto’s $300 million investment in Novozymes in their so-called BioAg Alliance

Dr. David G. Riley
Coastal Plain Exp. Stn.
Dept. Ent., P.O. Box 748
Tifton, GA 31793
Squash: Cucurbita pepo var. “Yellow Crookneck”
Pickleworm; Diaphania nitidalis (Stoll)
Cucumber beetles; Diabroticaspp.
Squash bugs; Anasa spp.
Melon aphid; Aphis gossypii
Stink Bugs
Sweetpotato whitefly, Bemisia tabaci (Gennadius)


Yellow crook neck squash was direct seeded into 2 rows per 6-ft bare ground beds on June 16 and maintained with standard cultural practices at the Lang Farm, Georgia Coastal Plain Experiment Station at Tifton. A total of 500 lbs/a of 10-10-10 was applied at planting to Tift pebbly clay loam field plots followed by two side-dress applications of 115 lbs/a 34-0-0. Irrigation was applied weekly with an overhead sprinkler system. Six foliar applications of insecticide were made on June 24, 30, July 2, 8, 17, and 22. Scouting was initiated on July 2 and continued weekly through harvest. One sample of 6 plants, with one leaf per plant for whitefly and aphid counts, was scouted per plot after weekly applications. Squash was harvested from 40 ft of row on July 21 and 30 and fruit were categorized as marketable, pickleworm damage, or virus damaged and the average weight was measured. Data was analyzed using GLM and LSD tests for separation of means (SAS Institute 1990).

The best treatments in terms of melon aphid control early in the test were the Tyratech A, QRD 416 2qt rate, and “SoySoap treatments” By the July 11, the NNI-0101 treatment had a delayed, but very strong effect on suppressing aphids while the other treatments were overwhelmed by a large aphid migration. Unfortunately, none of the treatments provided a strong reduction in the number of mosaic virus-affected squash fruit compared with the untreated check. Also, NNI-0101 tended to increase the incidence of pickleworm which may have offset the benefit of aphid control. Even so, NNI-0101 did provide the highest marketable yield followed by MOI-201 and the 2 qt rate of QRD416 in the first and second harvests. The aphid and pickleworm pressure during this test was very, causing the quality of squash from the check plot plants to be severely affected by the final harvest date (94% unmarketable). Most of the foliar treatments provided some benefit in terms of marketable squash, but under this heavy insect and virus disease pressure, more frequent sprays would have been required to improve yields. Most of the commercial cultivars of squash grown in southern Georgia during the summer include a transgenic resistance to mosaic viruses, but in this evaluation of bio-rational and/or organic treatments we used the standard open pollinated cultivar. In order to evaluate these treatments under a lower pressure scenario, an earlier spring planting would have to be conducted. One useful observation under this heavy insect pressure was that over all, none of the treatments significantly reduced the number of predatory arthropods.

Treatment – product rate per acre

Aphid 7/2/08

Predator1 7/11/08

Aphid 7/11/08

Leaf footed Bug 7/24/08

Stink Bugs 7/24/08

Predator overall

Aphids overall

1. Untreated Check

46 ab

2.3 abc

291 a

0.5 ab

1.3 ab

1.6 a

76 a

2. Novozymes MET @80 oz/a

33 bcd

1.5 abc

284 a

0.0 b

0.5 abc

2.3 a

73 a

3. Tyratech A @ 40 oz/a

28 d

1.3 bc

271 a

0.0 b

0.3 bc

1.8 a

69 a

4. Tyratech B @ 40 oz/a

47 a

1.5 abc

233 a

0.0 b

0.5 abc

1.7 a

63 a

5. AgraQuest, Inc. QRD 416 @ 2 qt/a

30 cd

1.3 bc

256 a

0.0 b

0.0 c

1.3 a

66 a

6. AgraQuest, Inc. QRD 416 @ 1 qt/a

42 abc

2.8 ab

331 a

0.8 a

1.0 abc

1.5 a

86 a

7. AgraQuest, Inc. QRD 416 @ 1 qt/a+ Knack @ 10 zo/a

34 abcd

3.5 a

254 a

0.3 ab

0.3 bc

2.2 a

68 a

Nichino America, Inc.
(pyrifluquinazon)@ 1.6 oz/a

40 abcd

0.3 c

74 b

3.3 ab

1.5 a

1.6 a

33 a

9. SoySoap 0.5% v/v

31 cd

2.3 abc

255 a

0.8 a

0.0 c

2.1 a

70 a

10. Marone MOI-201 @ 0.2% v/v

33 bcd

1.0 bc

299 a

0.3 ab

1.3 ab

2.4 a

75 a

* Means within columns followed by the same letter are not significantly different (LSD, P<0.05).

1Predators include various species of spiders and Coccinella sp.

Treatment – product rate per acre

Market wt 7/21/08

Virus fruit 7/30/08

Pickle Worm fruit wt 7/30/08

Total wt overall

Market wt overall

Pickle Worm wt overall

Virus wt overall

1. Untreated Check

0.5 b

7.8 c

1.3 b

9.0 a

0.36 b

3.2 abc

5.3 bc

2. Novozymes MET @80 oz/a

0.7 b

12.0 bc

2.3 ab

11.2 a

1.21 ab

4.6 abc

5.2 bc

3. Tyratech A @40 oz/a

0.7 b

14.3 abc

1.4 b

8.9 a

0.73 ab

2.6 c

5.8 bc

4. Tyratech B @ 40 oz/a

0.5 b

8.0 c

1.9 b

10.6 a

0.43 b

5.3 ab

5.2 bc

5. AgraQuest, Inc. QRD 416

@ 2 qts/a

1.4 ab

11.8 bc

1.8 b

11.1 a

1.34 ab

3.4 abc

6.2 abc

6. AgraQuest, Inc. QRD 416

@ 1 qt/a

0.6 b

10.0 bc

1.3 b

8.4 a

0.45 b

3.0 bc

4.9 c

7. AgraQuest, Inc. QRD 416

@ 1 qt/a+ Knack @ 10 oz/a

1.1 ab

18.0 ab

1.8 b

10.9 a

0.89 ab

2.2 c

7.9 a

Nichino America, Inc.
(pyrifluquinazon)@ 1.6 oz/a

2.2 a

6.8 c

3.5 a

12.1 a

1.75 a

5.6 a

5.2 bc

9. SoySoap 0.5% v/v

1.0 ab

8.8 bc

1.7 b

9.1 a

0.78 ab

4.0 abc

4.2 c

10. Marone MOI-201 @ 0.2% v/v

2.1 a

22.8 a

1.8 b

11.3 a

1.80 a

2.7 c

7.1 ab

We did very good beating companies that sold for hundreds of millions of dollars. Back than were not in the Pest Control Biopesticides business.
We plan to enter that business with our PicoAg 25B product in 2019 11 years after the above test.
Starting to look for trails with Universities, Governemnts and JV's with PicoAg 25B product.
This was our first and last attempt in 2008 at these biopseticide pests and we should of increased the rate to 5oz/10gallon acre application program, but did very well!
The Orange are beats 11 years ago with PicoAg 25B

* Means within columns followed by the same letter are not significantly different (LSD, P<0.05).

Biopesticides Primed for Growth By: Jackie Pucci | August 12, 2014

“Big things have small beginnings,” is the famous quote from classic flick Lawrence of Arabia. For biopesticides, the maxim holds true: They are confined to the fruit and vegetable fields no longer.

As more major multinationals have jumped into the biopesticides arena, more suppliers, and larger suppliers, mean wider distribution and deeper market penetration of naturally derived products in years to come – especially in coveted row crop areas like the U.S. Midwest and the Brazilian Cerrado.

“That [fruits and vegetables] was a great starting point,” Ziv Tirosh, CEO of Israel-based Stockton Group, maker of Timorex Gold biofungicide, said in an interview with Farm Chemicals International. “But the heart and soul of our food chain is row crops, and it’s a different ball game in terms of economics and application rates. Nevertheless, Stockton and other biopesticide companies are working hard at creating biopesticides that will work economically on row crops.”

We dont see these buys as Pico competition,The biologicals buying spree by agchem companies large and small swept the industry almost as fast as the spread of weed resistance. From Bayer’s trendsetting purchase of AgraQuest for nearly $500 million to BASF’s $1.02 billion acquisition of Becker Underwood to Monsanto’s $300 million investment in Novozymes in their so-called BioAg Alliance (to name a few), the value and potential of these products are not only being recognized but sought after. Twenty years ago, who could have imagined the current scenario?

For biopesticide companies, multinationals’ growing appetite for their products means immediate global market access and far greater resources to support product R&D, registration, manufacturing and marketing, among other prime opportunities. Easier regulatory also makes them attractive, with the typical timeframe being three to four years versus nine to 10 years, and not even 1/10 of the $250 million cost to register a traditional crop chemical.

“The interest of global crop protection companies to invest in biologicals will certainly enhance market acceptance and market penetration, especially in fruits and vegetables, but also in row crops, for example in the U.S. and Brazil,” said Utz Klages, Bayer CropScience spokesman.

Tirosh added, “There’s no doubt that the continued adoption of biopesticides by multinationals means that penetration into mainstream spray programs will continue at a rapid pace and clearly this will add to the exploration of value into row crops.”

None of this is to say that incorporating biopesticides is an automatic easy transition for traditional crop protection companies – far from it.

Challenges include biopesticides’ more demanding manufacturing and logistics, and the need to learn how to evaluate, develop and market the products, according to Dr. Mark Trimmer of the consultancy DunhamTrimmer. Training field staff is key. “Traditional crop protection companies will need to adjust their sales and marketing approaches to succeed with biologicals,” Trimmer said in an interview.

“Biopesticide benefits, such as residue and resistance management, are optimized when used in programs in combination with conventional chemistry,” he said. “Those companies that integrate biologicals into their thinking and train their field sales teams to promote them effectively will have an advantage.”

Big Growth and the ‘Wal-Mart Factor’

Bill Stoneman, executive director of the Biopesticides Industry Alliance, pointed out that it would seem that few biological companies would be left to acquire, but instead, he said more have sprouted up in the wake of the buying spree. Companies are also increasingly reaching out to seed treatment players to bulk up their portfolios and boost biopesticide consumption, such as Syngenta’s Clariva biological seed treatment nematicide based on technology it acquired from Pasteuria Bioscience in 2012.

Another recent example: In March, Bayer acquired Biagro Group, an Argentinian producer and distributor of biological seed treatment solutions especially in soybeans. Bayer is set to further expand its seed treatment business, known as SeedGrowth, by offering “an attractive and high-quality on-seed portfolio based on products, coatings, equipment and services,” said Matthias Haug, head of Bayer SeedGrowth.

Biopesticides still represent only about 3.5% or $1.93 billion of the $53 billion global crop protection market, according to DunhamTrimmer. That is up from $1 billion five years ago and $500 million a decade ago. The industry is highly fragmented, with more than 200 companies operating globally and the top 20 of those accounting for two-thirds of the market. Compare that with traditional crop protection market, in which the Big 6 eat up more than 72% of total sales.

Pam Marrone, founder and CEO of Marrone Bio Innovations

The biopesticide industry rose more than 15% last year, and the trend is expected to continue. Pamela Marrone, founder and CEO of Marrone Bio Innovations, said her company outpaced that growth with more than doubling of sales. “The growth drivers of using biologicals for residue and resistance management and where chemicals are restricted or not allowed, will continue,” she said. Further, she noted that biologicals can be used right up to harvest to manage residues, are produced using agricultural raw materials and aid in reducing water use in crop production. There is also the Wal-Mart factor: They can help large food companies and retailers meet their sustainability goals, and help meet consumers’ requirements for health and wellness.

The launches move along at a fast clip. Marrone is rolling out one to new products per year and expanding its existing products, including Grandevo bioinsecticide and Regalia biofungicide, which snapped up five new registrations in Latin America last year and ran a successful test launch for plant health in corn and soybeans last year. “We are expanding its acreage in 2014 and moving into canola, wheat and rice. We also found that Regalia’s mode of action for resistance management and bee safety gave it a boost in California almonds,” she said.

Following this spring’s debut of Venerate bioinsecticide, Marrone is also set to launch Haven, a product that reduces transpiration, resulting in crop yield increase. In less than a year, the company built a fermentation manufacturing plant for making Grandevo, and in June, it closed on $40 million follow-on stock offering. “These new funds allow us to accelerate moving our active ingredients into seed treatments, further international expansion and to expand the pipeline,” Marrone said.

Stockton Group’s Tirosh summed up the industry’s generally optimistic outlook: “We are still in the very initial era of penetration of biopesticides and their full adoption into spray programs … We have enough value already to make this into a solid shift.”

"PicoAg 4n1 25B" is a pico-biopesticide and Bacteria, Insects, Fungi, and Virus are controlled!

"PicoAg 4n1 25B" is a biopesticide and Bacteria, Insects, Fungi, and Virus are controlled!

We don't see these buys as Pico competition for last 20 years, The biologicals buying spree by agchem companies large and small swept the industry almost as fast as the spread of weed resistance. Bayer’s trendsetting purchase of AgraQuest for nearly $500 million to BASF’s $1.02 billion acquisition of Becker Underwood to Monsanto’s $300 million investment in Novozymes in their so-called BioAg Alliance

As a biopesticide you need a multipurpose mode of action for each elimination of vital elements in Bacteria, Insects, Fungi, and Virus pests you want to control.

Bacteria: elimination of cell membrane and to puncture it and drain proteins and lipid, PH.
Fungi: elimination of the cellulose and chitin.
Viruses: elimination of strands of nucleic acid, either DNA or RNA, and protective protein coat (the capsid), Or a lipid envelope, surrounding the protein.
Insects: elimination or penetration and dissolve lipid cellular membranes, cells desiccation, cellular metabolism, dissolving cuticles, lubrication joints leading to paralysis, stripping the pests protective shields, exoskeleton structure, chitin and protein substances, hydrocarbon chains smothering.

"PicoAg 4n1 25B" immediately impacts the exoskeleton structure of the pest upon contact by disrupting the molecular structure of the chitin and other protein substances that protect the insect. This mechanism of action triggers the rapid and irreversible deterioration of the insect's spiracles and tracheal system, resulting in suffocation. "PicoAg 4n1 25B" kills insects with elimination of chitin is a polysaccharide, a carbohydrate that has a chain sugar molecules, Chitin is a structure like cellulose. In addition to being found in exoskeletons.

"PicoAg 4n1 25B" major benefit of this revolutionary method of insect control is the absence of undesirable side effects on human health and no harm to the ecosystem. Additionally, unlike standard insecticides in use today, no built-in resistance can be developed by the targeted insects, but rather on the respiratory apparatus."

Science suggests that "PicoAg 4n1 25B" can be mechanical in primary sequential steps:

The first step is a direct interaction between the surface and the pests outer membrane, causing the membrane to rupture and leak fluids, proteins and nutrients.
  • There can be a second step related to the holes in the outer membrane, through which the pests lose vital nutrients, protein, water and components, causing a general weakening of the pests.
  • Lastly a few more ways "PicoAg 4n1 25B" electromechanical can affect pests
  • Electromechanical in can affect pests by penetration and dissolve lipid cellular membranes.
  • This causes cells desiccation to leak water, proteins and nutrients and collapse,
  • By interfering with cellular metabolism during metamorphosis,
  • By dissolving cuticles the lubrication in the insect’s joints leading to paralysis
  • By stripping the pests protective shields (wax, biofilm, etc), rendering it defenseless against subsequent treatment
  • The extracts impact the exoskeleton structure of pests upon contact by disrupting the molecular structure of the chitin and other protein substances that protect the insect,
  • The extracts have the ability to penetrate complex hydrocarbon chains and disintegrate them,
  • The extracts emulsify pests thus stopping their reproduction cycle.
  • The change the environment for growth with PH from acidophiles and neutrophiles to alkaliphiles .

    After punching holes, how does "PicoAg 4n1 25B" further damage the cell? Now that the cells main defense has been breached, there is an unopposed stream of "PicoAg 4n1 25B" entering the pest cell. This puts several vital processes inside the cell in danger. "PicoAg 4n1 25B" literally overwhelms the inside of the cell and obstructs cell metabolism (i.e., the biochemical reactions needed for life). These reactions are accomplished. When "PicoAg 4n1 25B" binds to these enzymes, their activity grinds to a halt. Pests can no longer "breathe", "eat", "digest", “reproduce” or “exist”.

    How can "PicoAg 4n1 25B" punch holes in a pests? Every cell's outer membrane, including that of a single cell organism like a pests, is characterized by a stable electrical micro-current. This is often called "transmembrane potential", and is literally, a voltage difference between the inside and the outside of a cell. It is strongly suspected that when a pests comes in contact with a "PicoAg 4n1 25B" surface, a short circuiting of the current in the cell membrane can occur. This weakens the membrane and creates holes and leak water, proteins and nutrients.

    How can "PicoAg 4n1 25B" effect be so fast, and affect such a wide range of pests? The experiences observed explain the speed with which pests and other pests perish on "PicoAg 4n1 25B" surfaces by the multi-targeted effects. After membrane perforation, can inhibit any given enzyme that "stands in its way," and stop the cell from transporting or digesting nutrients, from repairing its damaged membrane, from breathing or multiplying. Harmless to Environment Air, Water, Soil, Humans, Birds and Animals. This 80 year old science has no side effects or harm on human, birds and animal health. These solutions do not harm mammal cells nor do they attack neurological systems of humans, birds and animals.

    How Does "PicoAg 4n1 25B" Puncture And Leak From Membranes? It is used on lyse cells to extract protein or organelles, or to permeabilize the membranes of living cells.

    What is permeabilization of cells? The organic product dissolve lipids from cell membranes making them permeable to antibodies. Because the organic solvents also coagulate proteins, they can be used to fix and permeabilize cells at the same time. Saponin interacts with membrane cholesterol, selectively removing it and leaving holes in the membrane. Permeabilization is a the process of making something, such as a membrane or cell wall, permeable. Lyse is a verb referring to the process of lysis, the death of a cell. Lysis (/'la?s?s/ LY-sis; Greek ??s?? lýsis, "a loosing" from ??e?? lýein, "to unbind") refers to the breaking down of the membrane of a cell, often by viral, enzymic, or osmotic (that is, "lytic" /'l?t?k/ LIT-?k) mechanisms that compromise its integrity. A fluid containing the contents of lysed cells is called a lysate. In molecular biology, biochemistry, and cell biology laboratories, cell cultures may be subjected to lysis in the process of purifying their components, as in protein purification, DNA extraction, RNA extraction, or in purifying organelles.

    Trophobiosis Cycle: Pests shun healthy plants. Pesticides weaken plants. Weakened plants open the door to pests and disease. Hence pesticides precipitate pest attack and disease susceptibility, and thus they induce a cycle of further pesticide use. Unlike previous Biorationals, "PicoAg controls Bacteria, Fungi, Virus and Small Insects, so visit www.picocides.com !

    Picotechnology is a Game Changer for today's Agriculture Product “PicoAG 4-N-1" made of only atoms 1000 times smaller than nano and made of femtotechnology (Electrons, Protons, Neutrons) elements! PicoAg will replace Ag Pesticides, Ag Fertilizer, Ag Remediation, and Ag Production with No Side Effects with a single product of atoms 100% organic matter. So this begs the question why, isn't Picotechnology taught in any worldwide university?, Because there would be no Agri-Chem! Two universities say a technology change would cause millions of unemployed throughout agriculture industry! Don't forget the moisture in “PicoAG 4-N-1" can kill acidophiles, neutrophiles, The Purge has Started!!

    "PicoAG 4-N-1" product is made of Femtotechnology (Electrons, Protons, Neutrons)
    elements! In Just 2 oz you get this!

    In one inch you have 74,708,882 Atoms
    In one square inch of you have 5,580,968,805,397,920 Atoms
    In one cubic inch or 2 oz you have 416,931,197,021,738,000,000,000 Atoms

    Total Atoms Per Acre Atoms Per Foot Atoms Per Sq Inch
    Total Atoms 416,931,197,021,738,000,000,000 12,063,981,395,304,900,000 83,777,648,578,506,400
    The Carbon(C) 376,071,939,713,608,000,000,000 10,881,711,218,565,000,000 75,567,439,017,812,800

    Nitrogen (N) 33,354,495,761,739,100,000,000 965,118,511,624,394,000 6,702,211,886,280,520
    Phosphorus (P) 667,089,915,234,781,000,000 19,302,370,232,487,900 134,044,237,725,610
    Potassium (K) 958,941,753,149,998,000,000 27,747,157,209,201,300 192,688,591,730,565
    Calcium (Ca) 875,555,513,745,650,000,000 25,334,360,930,140,300 175,933,062,014,864
    Magnesium (Mg) 667,089,915,234,781,000,000 19,302,370,232,487,900 134,044,237,725,610
    Sulfur (Su) 625,396,795,532,607,000,000 18,095,972,092,957,400 125,666,472,867,760
    Zinc (Zn) 25,015,871,821,304,300,000 723,838,883,718,296 5,026,658,914,710
    Manganese (Mn) 50,031,743,642,608,600,000 1,447,677,767,436,590 10,053,317,829,421
    Iron (Fe) 75,047,615,463,912,900,000 2,171,516,651,154,890 15,079,976,744,131
    Copper (Cu) 4,169,311,970,217,380,000 120,639,813,953,049 837,776,485,785
    Boron (B) 16,677,247,880,869,500,000 482,559,255,812,197 3,351,105,943,140
    Sodium (Na) 250,158,718,213,043,000,000 7,238,388,837,182,960 50,266,589,147,104
    Chlorine (Cl) 500,317,436,426,086,000,000 14,476,777,674,365,900 100,533,178,294,208
    Molybdenum (Mo) 416,931,197,021,738,000,000 12,063,981,395,304,900 83,777,648,578,507
    Aluminum (Al) 488,893,521,627,690,000,000 14,146,224,584,134,600 98,237,670,723,157
    Silicon (SI) 300,857,551,770,886,000,000 8,705,368,974,852,040 60,453,951,214,250

    Pico Ag Med Cleaning Inc. 805 Cottage Hill Way, Brandon, Fl 33511 USA 800-995-9203, Intl 336-306-0193
    Email or Call: donwilshe@biobased.us

    20 Years making products from Atoms
    Applications for Pico-Technology Websites:

    Biobased USA
    Donald Wilshe
    805 Cottage Hill Way
    Brandon, Fl 33511
    Email: donwilshe@biobased.us
    Phone USA 800 995-9203 Direct to Cell.
    Phone USA INTL 336-306-0193